The hybrid Bohemian knotweed [Polygonum ×bohemicum (J. Chrtek & Chrtková) Zika & Jacobson [cuspidatum × sachalinense]; syn.: Reynoutria ×bohemica Chrtek & Chrtková] is part of the worldwide problematic rhizomatous invasive plants that impact (semi-)natural and agricultural systems. In this context, precise knowledge about the morpho-anatomy and resprouting capacity of the underground organs is key information for developing efficient eradication measures. In the present study, we aimed at (1) clarifying existing differences in the morpho-anatomical characteristics of rhizomes and roots, (2) developing an easy-to-apply field identification method for the underground organs, and (3) identifying the main morpho-anatomical features enhancing the rhizomes' resprouting ability. For this purpose, we collected the underground organs of two wild populations of P. ×bohemicum in Canton Ticino (southern Switzerland) and analyzed the morpho-anatomical differences between rhizomes and roots, using high-resolution microscope images and microtome sections. Collected material was then used for a resprouting capacity test after assessing rhizome characteristics such as weight, total diameter, pith diameter, pith brightness, and pith color. In contrast to roots, rhizomes are characterized by pith tissue in the center and display nodes with peripheral dormant buds that enable them to resprout. Resprouting ability of rhizomes was high (87.1% on average) and depended on the ontogenetic developmental stage of the organs (peak values of 97% for young and clearer-colored organs, 50% for old and dark ones). In conclusion, the smooth pith tissue of rhizomes represents a key discriminating feature between rhizomes and roots, whereas relating existing nodes to the corresponding rhizome pith color allows assessment of the resprouting potential of a knotweed population.